Annette de Kloet, Ph.D. Research.

Some specific research topics currently being explored include the impact of high-fat diet on angiotensin-sensitive neural circuits; the role of angiotensin-II and related peptides in the neuroendocrine regulation of energy balance; optogenetic targetting of angiotensin-sensitive neurons of the nodose ganglion to combat obesity and hypertension; mechanisms by which angiotensin-sensitive neurons of the median preoptic nucleus coordinate physiological and pathophysiological responses to hypertensive stimuli; mechanisms linking angiotensin-sensitive neurons and thermogenic responses to perturbations in energy balance and blood pressure regulation.

In order to explore these and other research topics, the de Kloet lab uses a multi-level approach that has spans the use of several molecular, genetic, physiological, and behavioral techniques.  Some examples of the approaches used in her laboratory are telemetry and indirect calorimetery, which are used to assess cardiovascular parameters and energy expenditure respectively; optogenetics, fiber photometry, neuronal tract tracing, mRNA in situ hybridization, and immunohistochemistry, which are used to characterize the structure and function of specific sets of neurons involved in these processes; and genetic recombination and virally-mediated gene transfer techniques, which allow for the manipulation of gene expression in specific cellular phenotypes.